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Becton dickinson simultest control y1/y2a (igg1 fitc/igg2a pe) is a two-color direct immuno-fluorescence reagent for use as a negative control in flow cytometic immunophenotyping of erythrocyte-lysed whole blood (lwb); simultest control y1/y2a is used with simultest software to set fluorescence-1 (fl1) and fluorescence-2 (fl2) quadrant markers around the unstained (negative) lymphocyte population and to estimate the amount of nonantigen-specific antibody binding (nonspecific staining) present, particulary that caused by fc receptors; bd simultest control y1/y2a is for in vitro diagnostic use with bd in vitro diagnostic simultest reagents; simultest control y1/y2a is a combination of two murine monoclonal antibodies, one conjugated with fitc and one conjugated with pe; the reagent, sufficient for 50 tests, is provided in 1.0 ml of phosphate-buffered saline (pbs) with gelatin and 0.1 % sodium azide

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